DNA Extraction of Blood The first step in molecular studies was to perform DNA extraction from the collected blood samples. For DNA extraction many different methods are used like manual method and through extraction kit. The DNA extraction from these samples was performed by using manual DNA extraction. Protocol for manual DNA extraction Day 1 1) The frozen blood is thaw at room temperature. 2) Take 200µl of blood in an eppendorf tube. Add 600µl of tris EDTA buffer in blood sample. 3) Mix the sample by inverting several times and then centrifuge it at 3000 rpm for 5 minutes. 4) Discard about 300 µl s...
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