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Tutorial Blast (NCBI)

  Object:   Starting with an organism and a protein, find a protein sequence and gene coding region.   Example:  Find the protein sequence and gene coding region for pathogenicity factor   listeriolysin O  from the bacterium  Listeria monocytogenes .     Searching for gene and protein information   Begin the search in  Gene , because it has less redundancy than Protein (this same search in Protein retrieves over 1000 records).   Search:   Listeria monocytogenes[organism] AND listeriolysin O[ protein name]   (see  Searchable fields in Gene )     One record, for gene symbol  hly , is retrieved. It is associated with an NC_ accession number (specifying a complete genomic molecule that is usually a reference assembly; see  RefSeq accession numbers and molecule types ).   To find the  gene coding sequence , look at the  Genomic regions, transcripts, and products  section or the  NCBI Reference Sequences ( RefSeq )   section of the Gene record:         Clicking on the   GenBan k  link disp