DECODE The Script of LIFE

   DNA Extraction of Blood The first step in molecular studies was to perform DNA extraction from the collected blood samples. For DNA extraction many different methods are used like manual method and through extraction kit. The DNA extraction from these samples was performed by using manual DNA extraction. Protocol for manual DNA extraction                                                                                                Day 1 1) The frozen blood is thaw at room temperature. 2) Take 200µl of blood in an eppendorf tube. Add 600µl of tris EDTA buffer in blood sample. 3) Mix the sample by inverting several times and then centrifuge it at 3000 rpm for 5 minutes. 4) Discard about 300 µl s...

  Object:   Starting with an organism and a protein, find a protein sequence and gene coding region.   Example:  Find the protein sequence and gene coding region for pathogenicity factor   listeriolysin O  from the bacterium  Listeria monocytogenes .     Searching for gene and protein information   Begin the search in  Gene , because it has less redundancy than Protein (this same search in Protein retrieves over 1000 records).   Search:   Listeria monocytogenes[organism] AND listeriolysin O[ protein name]   (see  Searchable fields in Gene )     One record, for gene symbol  hly , is retrieved. It is associated with an NC_ accession number (specifying a complete genomic molecule that is usually a reference assembly; see  RefSeq accession numbers and molecule types ).   To find the  gene coding sequence , look at the  Genomic regions, transcripts, and products  section or th...