Skip to main content

What's Chromosome?

 Chromosomes are molecules of DNA and proteins. They are located in the nucleus of the cell and their function is to carry genes. A gene is a part of DNA that contains code for the synthesis of a protein or an RNA molecule.

💡
gene = a segment of DNA that codes for a protein / an RNA molecule

You can think of genes as of recipes that you use in the kitchen to make various dishes. In the recipe, you'd find ingredients and steps that you need to follow to make your favourite meal. Genes are actually very similar and contain all the instructions on how to make a protein.

However, there are also parts of DNA that do not code for any protein or an RNA molecule. These noncoding segments are sometimes referred to as junk DNA. Even though these parts of DNA don't contain instructions on the synthesis of a protein, they are still very important, although their precise role remains unknown.

💡
junk DNA = a segment of DNA that does not code for any protein / RNA molecule

If you want to refer to both the coding and noncoding segments of the DNA, you can use the term genome, which is used to refer to all the genetic material in the organism.

💡
genome = all the genetic material in the organism

How many chromosomes do we have?

In humans, there are 24 types of chromosomes. There are 22 chromosomes that are numbered from 1 to 22 and these we call autosomes. Then, there are two sex chromosomes X and Y. As you probably know, men have one X chromosome and one Y chromosome. Women, on the other hand, have two X chromosomes. Human cells contain 2 copies of each chromosome - that means, 2 copies of chromosome 1, chromosome 2, etc., together making 46 chromosomes in total. There are, however, some cells that don't have follow this rule, e.g. germ cells, which only have 1 copy of each chromosome. A full set of these 46 chromosomes is called a karyotype.

💡
karyotype = a complete set of chromosomes
a woman's karyotype (simplified)

What's the structure of a chromosome?

A chromosome has two arms - a p arm and a q arm. Between the two arms, there is a structure called centromere, which is very important during mitosis. At each end of a chromosome is a telemore, which protects the ends of a chromosome and is responsible for their proper replication. Every chromosome consists of 1 molecule of DNA and proteins. Together, they form a centromere. Here, you can see two centromeres because we're looking at a picture of a chromosome after replication, in other words, a chromosome with 2 molecules of DNA.

structure of a chromosome

What are the different types of chromosomes?

Chromosomes vary in size and structure. For that reason, we distinguish different types of chromosomesMetacentric chromosomes have p a q arm of the same length. Submetacentric chromosomes have their p arm shorter than their q arm. Acrocentric chromosomes have very short p arm. Telocentric chromosomes have only q arms.

What are the basic structural units of a chromosome?

As we said, chromosomes consist of a single molecule of DNA and proteins that modify its structure. This complex of DNA and proteins is called chromatinChromatin fibre is also the basic subunit of a chromosome. Chromatin proteins can be divided into two classes: the histones and the nonhistone chromosomal proteins. Histones are very important and for that reason they are present in chromosomes in almost the same amount as the DNA. They form a specific structure of the chromatin, which is called a nucleosome. Nucleosome is a basic structural unit of the chromatin.

💡
chromatin = complex of DNA and proteins
💡
nucleosome = chromatin structural unit; consists of DNA and histones

We often describe the chromatin fibre as a series of beads on a string. The string is in fact the DNA and the bead is a nucleosome. Because the DNA connects the individual nucleosomes, it is also sometimes referred to as the linker DNA.

nucleosome

Every nucleosome consists of DNA that is wrapped around it and a complex of histones. It is, in fact, a complex of eight histone proteins - H2A, H2B, H3, and H4, all of them present in two molecules. This structure of eight histone proteins is sometimes referred to as the histone octamer.

structure of the nucleosome
💡
histone octamer = two molecules of histones H2A, H2B, H3, H4 that form the nucleosome

A series of nucleosomes forms the chromatin fibre. The histone that allows this structure to be formed is the H1 histone and its function is to put the individual nucleosomes close together.

The structure of the chromatin and its condensation can be adjusted and cells have several mechanisms to do so. One of these are chromatin-remodelling complexes, complexes of proteins that change the position of DNA wrapped around the nucleosome making the DNA more or less accessible to other proteins in the cell.

Cells can also chemically modify histones to change the structure of the chromatin again making the DNA more or less accessible.

The consequence of these mechanisms is that the structure of the chromatin is not the same in every region of the chromosome. Parts of chromosomes that contain genes that are being expressed are more extended, while regions of chromosomes that contain genes that are not being expressed are more condensed (i.e., less extended).

Regions of the chromosome that are highly condensed are called heterochromatin. Heterochromatin is usually concentrated around the centromere and in the telomeres.

Regions of the chromosomes that are less condensed are called euchromatin. As we already said, if we want to express a gene, we want its DNA extended, which means that DNA in the heterochromatin is usually not expressed.

💡
heterochromatin = highly condensed regions of the chromatin
💡
euchromatin = less condensed regions of the chromatin

Comments

Popular posts from this blog

 Genomics_command_line_quiz1 For all projects, you may use your own Unix-based system and, where applicable, ensure that you are running the version of the software specified in the assignments. Alternatively, you may use the VMBox virtual machine environment provided with the course materials. Instructions on how to download and use the environment can be found on the course web site. For the following questions, refer to the class workflow and use the data in the Online materials (‘gencommand_proj1_data.tar.gz’) to answer the questions. Assume you sequenced and assembled the genome of Malus domestica (apple), and performed gene annotation. You then collected samples and ran RNA-seq experiments to determine sets of genes that are expressed in the various tissues. This information was stored, respectively, in the following files: “apple.genome”, “apple.genes”, “apple.condition{A,B,C}”. NOTE: The apple genome and the apple gene annotations for this project were extracted from the Rosace

Immunotherapy

 

Introduction to Molecular Biology

 Introduction to Molecular Biology Cells are fundamental building blocks of living organisms. Cells contain a nucleus, mitochondria and chloroplasts, endoplasmic reticulum, ribosomes, vacuoles, etc.  The nucleus is important organelle because it houses chromosomes which include the DNA.  The DNA is in essence a blueprint of the organism as it encodes information needed to synthesize proteins . Molecular biologist s would like to understand how human biology works with the hope to treat diseases like cancer. One can look at simpler organisms such as yeasts to understand how human biology works.  Admittedly, unicellular yeasts are very different from humans who have approximately 1014 cells. However, the DNA is similar across all living organisms. For example, humans share 99% of DNA with chimps. Naturally, we would like to know what information contained in that 1% of DNA is so critical to determine all the distinguishing features of humans,  DNA            DNA stands for deoxyribonucle